Cardiopathology provides insights into the functioning heart and its circulatory vessels. Recent studies have proposed a critical role for esterases in heart failure (Dunlop et. al. 2003). Evidence points to a loss of vagal (parasympathetic) control and a parallel increase in sympathetic activity as the disease progresses. Other researchers (Ojaimi et.al. 2007) used microarray technique on tissues from canine paced heart failure models to show selective re-regulation of genes as the pathology developed. To further explore this connection, a pilot project comparing the enzyme acetylcholinesterase (AChE) from normal (N) and heart failure (HF) specimens was designed. Non-denaturing polyacrylamide gel electrophoresis (PAGE) separated the isoenzyme components. Diazonium salts stained and identified the resulting enzyme/substrate banding patterns. The present study refines previous work by examining AChE activity of non-denatured protein from N and HF tissues. In addition, a variety of non-cardiac tissues and organs were examined to determine the extent of pathological changes in AChE isoenzymes.
Palmer, Cherelle and Blando-Hoegler, Charlene, "Acetylcholinesterase Isozyme Banding May Be Useful in Revealing Heart Failure" (2012). Student-Faculty Research Projects. Paper 3.